Blocking Assay:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
Purification:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
Virus:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
Recombinant:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
cDNA Synthesis:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
RNA sequencing:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
Software:Article Title: The quality of energy- and macronutrient-balanced diets regulates host susceptibility to influenza in mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Fc Block (2.4G2) BD 553142; RRID: AB_394657 CD4 - AF700 (RM4-5) BD 557956; RRID: AB_396956 CD8 - BV711 (53-6.7) BD 563046; RRID: AB_2737972 CD44 – BV605 (IM7) BD 563058; RRID: AB_2737979 IFN-g - PE/Cy7 (XMG1.2) BD 561040; RRID: AB_396766 TNF-a - PerCP/Cy5.5 (MP6-XT22) BioLegend 506321; RRID: AB_961435 PE-conjugated PA224-233:H2-D b tetramer NIH N/A Purified NA/LE Anti-CD3e (145-2C11) BD 553057; RRID: AB_394590 Bacterial and virus strains PR8: A/PR/8/34 (H1N1) Common passaged lab strain - grown in house N/A Chemicals, peptides, and recombinant proteins UV Live/Dead Thermo Fisher Cat# L34962 RNAlater Sigma Aldrich Cat# R0901-500ML Trisure Bioline Cat# BIO-38033 DNase I Sigma Aldrich Cat# DN25-100MG; Cas: 9003-98-9 L-NMMA Cayman Chemical 10005031 ; RRID: 53308-83-1 L-15 Media Sigma Aldrich Cat# L4386-10X1L Avicel Sigma Aldrich 11365; Cas: 9004-34-6 Glucose Sigma Aldrich G8270-100G; Cas: 50-99-7 Insulin (Actrapid) ThePharmacy NA Pyruvate Sigma Aldrich P2256; Cas: 113-24-6 EDTA Thermo Fisher Cat# 15575020 T3 (3,305-Triiodo-L-thyronine) Cayman Chemical 17598; Cas: 5817-39-0 Trypsin Worthington Biochemicals Cat# LS003740 Collagenase type IV Sigma Aldrich C5138; Cas: 9001-12-1 Collagenase type II Sigma Aldrich C6885; Cas: 9001-12-1 Fixation/Permeabilization Kit BD 554714; RRID: AB_2869008 Critical commercial assays LegendPlex Mouse Type I/II IFN Panel BioLegend Cat# 740635 Tetro cDNA synthesis Kit Bioline Cat# BIO-65043 SYBR No-ROX Master Mix Bioline Cat# BIO-98020 Deposited data RNAseq data This paper GEO: GSE215976 Experimental models: Cell lines MDCK Common cell line – grown in house N/A Experimental models: Organisms/strains C57BL/6J Australian BioResources N/A Ifngr1 / (B6.129S7-Ifngr1tm1Agt/J) The Jackson Laboratory 003288 Oligonucleotides See Table S3 for oligonucleotide sequences N/A N/A Software and algorithms GraphPad Prism 8 GraphPad Software RRID: SCR_002798 MetaScreen Sable Systems https://www.sablesys.com (Continued on next page) e1 Cell Reports 41, 111638, November 15, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER ImageScope Leica Biosystems https://www.leicabiosystems.com RStudio RStudio https://www.rstudio.com FACSDiva Software BD https://www.bdbiosciences.com FlowJo10 TreeStar https://www.flowjo.com QuPath Queen’s University https://qupath.github.io Other Irradiated chow diet (Meat Free Rat and Mouse Diet) Specialty Feeds https://www.specialtyfeeds.com/new/ wp-content/uploads/2016/06/irr_rm.pdf Irradiated AIN93G diet + extra vitamins Specialty Feeds SF09-091 Promethion behavioral phenotyping cages Sable Systems Multiple Rectal thermometer Physitemp Instruments N/A Leica Aperio XT slide scanner Leica Biosystems https://www.leicabiosystems.com Roche LightCycler480 Roche https://diagnostics.roche.com NanoDrop 2000c Thermo Fisher Scientific Cat# ND-2000 Illumina HiSeq 2000 Illumina https://www.illumina.com BD Fortessa BD https://www.bdbiosciences.com EchoMRI-900 EchoMRI LCC http://www.echomri.com Glucometer Abbott N/A
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